Generation of KCL026 research grade human embryonic stem cell line carrying a mutation in SMN1 gene

Generation of KCL017 research grade human embryonic stem cell line carrying a mutation in VHL gene

The KCL017 human embryonic stem cell line was derived from an embryo donated for research that carried an autosomal dominant mutation affecting splicing site of the VHL gene encoding von Hippel-Lindau tumor suppressor E3 ubiquitin protein ligase (676+3A>T). The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line prop...

Derivation of the clinical grade human embryonic stem cell line RCe018-A (RC-14).

The human embryonic stem cell line RCe018-A (RC-14) was derived under quality assured compliance with UK regulation, European Union Directives and International guidance for tissue procurement, processing and storage according to Good Manufacturing Practice (GMP) standards. The cell line was derived from a blastocyst stage embryo voluntarily donated as unsuitable or surplus to fertility require...

Fellowships: Human Embryonic Stem Cell Research

Bonn Risk Index (BRI) is being used for the assessment of urinary calcium oxalate (CaOx) crystallization. There are no published data regarding BRI during growth. The objective of this study was to establish ageand sex-dependent BRI values in healthy children and adolescents. A total of 1,050 Caucasian subjects aged 3– 18 years (525 males, 525 females) without a history of kidney stone disease ...

The generation of six clinical-grade human embryonic stem cell lines.

Generation of human embryonic stem cell line expressing a red fluorescent protein: BJNhem20-pCAG-tdTomato.

Human embryonic stem cell line BJNhem20-pCAG-tdTomato was generated using non-viral method. The construct pCAG-tdTomato was transfected using microporation procedure. This fluorescent hESC line can help to study heterogeneity within individual cells in hESC colonies by enabling live tracking of their growth, migration and differentiation properties. This cell line also serves as a resource for ...